Construct: shRNA TRCN0000295355

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024287.3-487s21c1
DNA Barcode:
ATCCGCTGAGGAAATTCAAGC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rab6a (19346)
Gene Description:
RAB6A, member RAS oncogene family
Transcript:
RefSeq NM_024287.3 (NON-CURRENT)
Match location:
Position 487 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000295355)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATCCGCTGAGGAAATTCAAGC
Hairpin Sequence:
5'-CCGG-ATCCGCTGAGGAAATTCAAGC-CTCGAG-GCTTGAATTTCCTCAGCGGAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATCCGCTGAGGAAATTCAAGCCTCGAGGCTTGAATTTCCTCAGCGGATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATCCGCTGAGGAAATTCAAGCCTCGAGGCTTGAATTTCCTCAGCGGAT-3'

Other clones with same target sequence:

(none)