Construct: shRNA TRCN0000295441

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145076.3-1101s21c1
DNA Barcode:
AGTGATCATAGATACTCTAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Trim24 (21848)
Gene Description:
tripartite motif-containing 24
Transcript:
RefSeq NM_145076.3 (NON-CURRENT)
Match location:
Position 1101 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000295441)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGTGATCATAGATACTCTAAT
Hairpin Sequence:
5'-CCGG-AGTGATCATAGATACTCTAAT-CTCGAG-ATTAGAGTATCTATGATCACT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGTGATCATAGATACTCTAATCTCGAGATTAGAGTATCTATGATCACTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGTGATCATAGATACTCTAATCTCGAGATTAGAGTATCTATGATCACT-3'

Other clones with same target sequence:

(none)