Construct: shRNA TRCN0000295462
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_027175.3-1273s21c1
- DNA Barcode:
- TCAGTAGAGTAGTGGTCACAA
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Ndufaf1 (69702)
- Gene Description:
- NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, assembly factor 1
- Transcript:
- RefSeq NM_027175.3 (CURRENT)
- Match location:
- Position 1273 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000295462)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- TCAGTAGAGTAGTGGTCACAA
- Hairpin Sequence:
-
5'-CCGG-TCAGTAGAGTAGTGGTCACAA-CTCGAG-TTGTGACCACTACTCTACTGA-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGTCAGTAGAGTAGTGGTCACAACTCGAGTTGTGACCACTACTCTACTGATTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAATCAGTAGAGTAGTGGTCACAACTCGAGTTGTGACCACTACTCTACTGA-3'
Other clones with same target sequence:
(none)
