Construct: shRNA TRCN0000295583

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019725.1-899s21c1
DNA Barcode:
GAAGACAAGAGTGACTATAAC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tle2 (21886)
Gene Description:
transducin-like enhancer of split 2
Transcript:
RefSeq NM_019725.1 (NON-CURRENT)
Match location:
Position 899 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000295583)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGACAAGAGTGACTATAAC
Hairpin Sequence:
5'-CCGG-GAAGACAAGAGTGACTATAAC-CTCGAG-GTTATAGTCACTCTTGTCTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGACAAGAGTGACTATAACCTCGAGGTTATAGTCACTCTTGTCTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGACAAGAGTGACTATAACCTCGAGGTTATAGTCACTCTTGTCTTC-3'

Other clones with same target sequence:

(none)