Construct: shRNA TRCN0000295598
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_008186.4-1558s21c1
- DNA Barcode:
- CAGATGGTGCCAAACGATATT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Gtf2h1 (14884)
- Gene Description:
- general transcription factor II H, polypeptide 1
- Transcript:
- RefSeq NM_008186.4 (CURRENT)
- Match location:
- Position 1558 (CDS)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000295598)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CAGATGGTGCCAAACGATATT
- Hairpin Sequence:
-
5'-CCGG-CAGATGGTGCCAAACGATATT-CTCGAG-AATATCGTTTGGCACCATCTG-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCAGATGGTGCCAAACGATATTCTCGAGAATATCGTTTGGCACCATCTGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACAGATGGTGCCAAACGATATTCTCGAGAATATCGTTTGGCACCATCTG-3'
Other clones with same target sequence:
(none)
