Construct: shRNA TRCN0000295607

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177698.4-976s21c1
DNA Barcode:
CATCGGTCCCAGATAACTAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Psd3 (234353)
Gene Description:
pleckstrin and Sec7 domain containing 3
Transcript:
RefSeq NM_177698.4 (CURRENT)
Match location:
Position 976 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000295607)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATCGGTCCCAGATAACTAAT
Hairpin Sequence:
5'-CCGG-CATCGGTCCCAGATAACTAAT-CTCGAG-ATTAGTTATCTGGGACCGATG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATCGGTCCCAGATAACTAATCTCGAGATTAGTTATCTGGGACCGATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATCGGTCCCAGATAACTAATCTCGAGATTAGTTATCTGGGACCGATG-3'

Other clones with same target sequence:

(none)