Construct: shRNA TRCN0000295608

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145470.2-1629s21c1
DNA Barcode:
GTTTAGAGTTCTCAATCATTG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Deptor (97998)
Gene Description:
DEP domain containing MTOR-interacting protein
Transcript:
RefSeq NM_145470.2 (NON-CURRENT)
Match location:
Position 1629 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000295608)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTTTAGAGTTCTCAATCATTG
Hairpin Sequence:
5'-CCGG-GTTTAGAGTTCTCAATCATTG-CTCGAG-CAATGATTGAGAACTCTAAAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTTTAGAGTTCTCAATCATTGCTCGAGCAATGATTGAGAACTCTAAACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTTTAGAGTTCTCAATCATTGCTCGAGCAATGATTGAGAACTCTAAAC-3'

Other clones with same target sequence:

(none)