Construct: shRNA TRCN0000295642

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016698.1-2918s21c1
DNA Barcode:
TCCATCGGGTTTCCTCTCTGT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rnf10 (50849)
Gene Description:
ring finger protein 10
Transcript:
RefSeq NM_016698.1 (NON-CURRENT)
Match location:
Position 2918 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000295642)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCCATCGGGTTTCCTCTCTGT
Hairpin Sequence:
5'-CCGG-TCCATCGGGTTTCCTCTCTGT-CTCGAG-ACAGAGAGGAAACCCGATGGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCCATCGGGTTTCCTCTCTGTCTCGAGACAGAGAGGAAACCCGATGGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCCATCGGGTTTCCTCTCTGTCTCGAGACAGAGAGGAAACCCGATGGA-3'

Other clones with same target sequence:

(none)