Construct: shRNA TRCN0000295666
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_027764.2-2044s21c1
- DNA Barcode:
- TGCGGTGATCTGCAGGTAAAG
Original Target:
- Taxon:
- Mus musculus (mouse)
- Gene:
- Rcbtb1 (71330)
- Gene Description:
- regulator of chromosome condensation (RCC1) and BTB (POZ) domain containing protein 1
- Transcript:
- RefSeq NM_027764.2 (CURRENT)
- Match location:
- Position 2044 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this shRNA:
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Sequence Information
- Target Sequence:
- TGCGGTGATCTGCAGGTAAAG
- Hairpin Sequence:
- 5'-CCGG-TGCGGTGATCTGCAGGTAAAG-CTCGAG-CTTTACCTGCAGATCACCGCA-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGTGCGGTGATCTGCAGGTAAAGCTCGAGCTTTACCTGCAGATCACCGCATTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAATGCGGTGATCTGCAGGTAAAGCTCGAGCTTTACCTGCAGATCACCGCA-3'