Construct: shRNA TRCN0000295779

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011678.2-3145s21c1
DNA Barcode:
ATGTTAAGAGGCTGGATTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Usp4 (22258)
Gene Description:
ubiquitin specific peptidase 4 (proto-oncogene)
Transcript:
RefSeq NM_011678.2 (CURRENT)
Match location:
Position 3145 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000295779)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATGTTAAGAGGCTGGATTATT
Hairpin Sequence:
5'-CCGG-ATGTTAAGAGGCTGGATTATT-CTCGAG-AATAATCCAGCCTCTTAACAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATGTTAAGAGGCTGGATTATTCTCGAGAATAATCCAGCCTCTTAACATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATGTTAAGAGGCTGGATTATTCTCGAGAATAATCCAGCCTCTTAACAT-3'

Other clones with same target sequence:

(none)