Construct: shRNA TRCN0000295839

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133259.3-4383s21c1
DNA Barcode:
GACCATAACTCTGTGCACTTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LRPPRC (10128)
Gene Description:
leucine rich pentatricopeptide repeat containing
Transcript:
RefSeq NM_133259.3 (NON-CURRENT)
Match location:
Position 4383 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000295839)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACCATAACTCTGTGCACTTG
Hairpin Sequence:
5'-CCGG-GACCATAACTCTGTGCACTTG-CTCGAG-CAAGTGCACAGAGTTATGGTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACCATAACTCTGTGCACTTGCTCGAGCAAGTGCACAGAGTTATGGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACCATAACTCTGTGCACTTGCTCGAGCAAGTGCACAGAGTTATGGTC-3'

Other clones with same target sequence:

  1. TRCN0000010373