Construct: shRNA TRCN0000295926

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025164.3-4148s21c1
DNA Barcode:
TTTCTTCCAGAGGTGATATAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SIK3 (23387)
Gene Description:
SIK family kinase 3
Transcript:
RefSeq NM_025164.3 (NON-CURRENT)
Match location:
Position 4148 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000295926)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTTCTTCCAGAGGTGATATAC
Hairpin Sequence:
5'-CCGG-TTTCTTCCAGAGGTGATATAC-CTCGAG-GTATATCACCTCTGGAAGAAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTTCTTCCAGAGGTGATATACCTCGAGGTATATCACCTCTGGAAGAAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTTCTTCCAGAGGTGATATACCTCGAGGTATATCACCTCTGGAAGAAA-3'

Other clones with same target sequence:

(none)