Construct: shRNA TRCN0000295939

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018218.2-2361s21c1
DNA Barcode:
GGTGTTTGATATTCGAATTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
USP40 (55230)
Gene Description:
ubiquitin specific peptidase 40
Transcript:
RefSeq NM_018218.2 (NON-CURRENT)
Match location:
Position 2361 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000295939)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGTGTTTGATATTCGAATTAA
Hairpin Sequence:
5'-CCGG-GGTGTTTGATATTCGAATTAA-CTCGAG-TTAATTCGAATATCAAACACC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGTGTTTGATATTCGAATTAACTCGAGTTAATTCGAATATCAAACACCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGTGTTTGATATTCGAATTAACTCGAGTTAATTCGAATATCAAACACC-3'

Other clones with same target sequence:

(none)