Construct: shRNA TRCN0000296064

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005324.3-809s21c1
DNA Barcode:
GACTTGTTGGGTAGCTATTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
H3-3B (3021)
Gene Description:
H3.3 histone B
Transcript:
RefSeq NM_005324.3 (NON-CURRENT)
Match location:
Position 809 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000296064)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACTTGTTGGGTAGCTATTAA
Hairpin Sequence:
5'-CCGG-GACTTGTTGGGTAGCTATTAA-CTCGAG-TTAATAGCTACCCAACAAGTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACTTGTTGGGTAGCTATTAACTCGAGTTAATAGCTACCCAACAAGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACTTGTTGGGTAGCTATTAACTCGAGTTAATAGCTACCCAACAAGTC-3'

Other clones with same target sequence:

(none)