Construct: shRNA TRCN0000296070

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032124.4-1328s21c1
DNA Barcode:
TTAGGCTGTAGTTCTAATATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HDHD2 (84064)
Gene Description:
haloacid dehalogenase like hydrolase domain containing 2
Transcript:
RefSeq NM_032124.4 (NON-CURRENT)
Match location:
Position 1328 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000296070)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTAGGCTGTAGTTCTAATATT
Hairpin Sequence:
5'-CCGG-TTAGGCTGTAGTTCTAATATT-CTCGAG-AATATTAGAACTACAGCCTAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTAGGCTGTAGTTCTAATATTCTCGAGAATATTAGAACTACAGCCTAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTAGGCTGTAGTTCTAATATTCTCGAGAATATTAGAACTACAGCCTAA-3'

Other clones with same target sequence:

(none)