Construct: shRNA TRCN0000296088

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003895.2-4817s21c1
DNA Barcode:
GAGAATTGGGCATTAGTATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SYNJ1 (8867)
Gene Description:
synaptojanin 1
Transcript:
RefSeq NM_003895.2 (NON-CURRENT)
Match location:
Position 4817 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000296088)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGAATTGGGCATTAGTATTT
Hairpin Sequence:
5'-CCGG-GAGAATTGGGCATTAGTATTT-CTCGAG-AAATACTAATGCCCAATTCTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGAATTGGGCATTAGTATTTCTCGAGAAATACTAATGCCCAATTCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGAATTGGGCATTAGTATTTCTCGAGAAATACTAATGCCCAATTCTC-3'

Other clones with same target sequence:

(none)