Construct: shRNA TRCN0000296190

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000610.3-1851s21c1
DNA Barcode:
ATGGACTCCAGTCATAGTATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CD44 (960)
Gene Description:
CD44 molecule (Indian blood group)
Transcript:
RefSeq NM_000610.3 (NON-CURRENT)
Match location:
Position 1851 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000296190)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATGGACTCCAGTCATAGTATA
Hairpin Sequence:
5'-CCGG-ATGGACTCCAGTCATAGTATA-CTCGAG-TATACTATGACTGGAGTCCAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATGGACTCCAGTCATAGTATACTCGAGTATACTATGACTGGAGTCCATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATGGACTCCAGTCATAGTATACTCGAGTATACTATGACTGGAGTCCAT-3'

Other clones with same target sequence:

(none)