Construct: shRNA TRCN0000296192

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024779.4-785s21c1
DNA Barcode:
CCTCAAGGGTTCCCTAGTGTC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PIP4K2C (79837)
Gene Description:
phosphatidylinositol-5-phosphate 4-kinase type 2 gamma
Transcript:
RefSeq NM_024779.4 (NON-CURRENT)
Match location:
Position 785 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000296192)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCAAGGGTTCCCTAGTGTC
Hairpin Sequence:
5'-CCGG-CCTCAAGGGTTCCCTAGTGTC-CTCGAG-GACACTAGGGAACCCTTGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCAAGGGTTCCCTAGTGTCCTCGAGGACACTAGGGAACCCTTGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCAAGGGTTCCCTAGTGTCCTCGAGGACACTAGGGAACCCTTGAGG-3'

Other clones with same target sequence:

  1. TRCN0000199085