Construct: shRNA TRCN0000296232

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_139015.3-2105s21c1
DNA Barcode:
GAAGCTCTTCTGTCGTTATAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SPPL3 (121665)
Gene Description:
signal peptide peptidase like 3
Transcript:
RefSeq NM_139015.3 (NON-CURRENT)
Match location:
Position 2105 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000296232)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGCTCTTCTGTCGTTATAT
Hairpin Sequence:
5'-CCGG-GAAGCTCTTCTGTCGTTATAT-CTCGAG-ATATAACGACAGAAGAGCTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGCTCTTCTGTCGTTATATCTCGAGATATAACGACAGAAGAGCTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGCTCTTCTGTCGTTATATCTCGAGATATAACGACAGAAGAGCTTC-3'

Other clones with same target sequence:

(none)