Construct: shRNA TRCN0000296261

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006825.3-609s21c1
DNA Barcode:
CTTGAGAAGCTCCCAACATAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CKAP4 (10970)
Gene Description:
cytoskeleton associated protein 4
Transcript:
RefSeq NM_006825.3 (NON-CURRENT)
Match location:
Position 609 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000296261)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTGAGAAGCTCCCAACATAA
Hairpin Sequence:
5'-CCGG-CTTGAGAAGCTCCCAACATAA-CTCGAG-TTATGTTGGGAGCTTCTCAAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTGAGAAGCTCCCAACATAACTCGAGTTATGTTGGGAGCTTCTCAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTGAGAAGCTCCCAACATAACTCGAGTTATGTTGGGAGCTTCTCAAG-3'

Other clones with same target sequence:

(none)