Construct: shRNA TRCN0000296373

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_012305.2-2824s21c1
DNA Barcode:
TGATCCTAATCCTGCGAATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
AP2A2 (161)
Gene Description:
adaptor related protein complex 2 subunit alpha 2
Transcript:
RefSeq NM_012305.2 (NON-CURRENT)
Match location:
Position 2824 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000296373)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGATCCTAATCCTGCGAATTT
Hairpin Sequence:
5'-CCGG-TGATCCTAATCCTGCGAATTT-CTCGAG-AAATTCGCAGGATTAGGATCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGATCCTAATCCTGCGAATTTCTCGAGAAATTCGCAGGATTAGGATCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGATCCTAATCCTGCGAATTTCTCGAGAAATTCGCAGGATTAGGATCA-3'

Other clones with same target sequence:

(none)