Construct: shRNA TRCN0000296382

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001002857.1-1445s21c1
DNA Barcode:
ACTTTAGAAACACGTACTTTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ANXA2 (302)
Gene Description:
annexin A2
Transcript:
RefSeq NM_001002857.1 (NON-CURRENT)
Match location:
Position 1445 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000296382)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACTTTAGAAACACGTACTTTG
Hairpin Sequence:
5'-CCGG-ACTTTAGAAACACGTACTTTG-CTCGAG-CAAAGTACGTGTTTCTAAAGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACTTTAGAAACACGTACTTTGCTCGAGCAAAGTACGTGTTTCTAAAGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACTTTAGAAACACGTACTTTGCTCGAGCAAAGTACGTGTTTCTAAAGT-3'

Other clones with same target sequence:

(none)