Construct: shRNA TRCN0000296437

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003692.3-293s21c1
DNA Barcode:
CATCAACTGCTCAGAATTAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TMEFF1 (8577)
Gene Description:
transmembrane protein with EGF like and two follistatin like domains 1
Transcript:
RefSeq NM_003692.3 (NON-CURRENT)
Match location:
Position 293 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000296437)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATCAACTGCTCAGAATTAAA
Hairpin Sequence:
5'-CCGG-CATCAACTGCTCAGAATTAAA-CTCGAG-TTTAATTCTGAGCAGTTGATG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATCAACTGCTCAGAATTAAACTCGAGTTTAATTCTGAGCAGTTGATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATCAACTGCTCAGAATTAAACTCGAGTTTAATTCTGAGCAGTTGATG-3'

Other clones with same target sequence:

(none)