Construct: shRNA TRCN0000296480

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004341.3-6910s21c1
DNA Barcode:
GGGCAGCACACTTAGATATTC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CAD (790)
Gene Description:
carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase
Transcript:
RefSeq NM_004341.3 (NON-CURRENT)
Match location:
Position 6910 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000296480)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGGCAGCACACTTAGATATTC
Hairpin Sequence:
5'-CCGG-GGGCAGCACACTTAGATATTC-CTCGAG-GAATATCTAAGTGTGCTGCCC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGGCAGCACACTTAGATATTCCTCGAGGAATATCTAAGTGTGCTGCCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGGCAGCACACTTAGATATTCCTCGAGGAATATCTAAGTGTGCTGCCC-3'

Other clones with same target sequence:

(none)