Construct: shRNA TRCN0000296539

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032486.2-842s21c1
DNA Barcode:
ACAGTCACTTTGTACCATTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DCTN5 (84516)
Gene Description:
dynactin subunit 5
Transcript:
RefSeq NM_032486.2 (NON-CURRENT)
Match location:
Position 842 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000296539)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACAGTCACTTTGTACCATTAT
Hairpin Sequence:
5'-CCGG-ACAGTCACTTTGTACCATTAT-CTCGAG-ATAATGGTACAAAGTGACTGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACAGTCACTTTGTACCATTATCTCGAGATAATGGTACAAAGTGACTGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACAGTCACTTTGTACCATTATCTCGAGATAATGGTACAAAGTGACTGT-3'

Other clones with same target sequence:

(none)