Construct: shRNA TRCN0000296662

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002309.3-715s21c1
DNA Barcode:
CTTCTAGCAGGAGGTCTTGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LIF (3976)
Gene Description:
LIF interleukin 6 family cytokine
Transcript:
RefSeq NM_002309.3 (NON-CURRENT)
Match location:
Position 715 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000296662)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTCTAGCAGGAGGTCTTGAA
Hairpin Sequence:
5'-CCGG-CTTCTAGCAGGAGGTCTTGAA-CTCGAG-TTCAAGACCTCCTGCTAGAAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTCTAGCAGGAGGTCTTGAACTCGAGTTCAAGACCTCCTGCTAGAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTCTAGCAGGAGGTCTTGAACTCGAGTTCAAGACCTCCTGCTAGAAG-3'

Other clones with same target sequence:

(none)