Construct: shRNA TRCN0000296747

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002061.2-840s21c1
DNA Barcode:
CCAAATAGTAACCAAGTTAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GCLM (2730)
Gene Description:
glutamate-cysteine ligase modifier subunit
Transcript:
RefSeq NM_002061.2 (NON-CURRENT)
Match location:
Position 840 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000296747)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAAATAGTAACCAAGTTAAT
Hairpin Sequence:
5'-CCGG-CCAAATAGTAACCAAGTTAAT-CTCGAG-ATTAACTTGGTTACTATTTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAAATAGTAACCAAGTTAATCTCGAGATTAACTTGGTTACTATTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAAATAGTAACCAAGTTAATCTCGAGATTAACTTGGTTACTATTTGG-3'

Other clones with same target sequence:

(none)