Construct: shRNA TRCN0000296760

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019001.3-5574s21c1
DNA Barcode:
ACACTATCATTGAGGAATAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
XRN1 (54464)
Gene Description:
5'-3' exoribonuclease 1
Transcript:
RefSeq NM_019001.3 (NON-CURRENT)
Match location:
Position 5574 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000296760)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACACTATCATTGAGGAATAAT
Hairpin Sequence:
5'-CCGG-ACACTATCATTGAGGAATAAT-CTCGAG-ATTATTCCTCAATGATAGTGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACACTATCATTGAGGAATAATCTCGAGATTATTCCTCAATGATAGTGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACACTATCATTGAGGAATAATCTCGAGATTATTCCTCAATGATAGTGT-3'

Other clones with same target sequence:

(none)