Construct: shRNA TRCN0000296800

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000135.2-1834s21c1
DNA Barcode:
GACTCCCGTGTGGCGTTTATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FANCA (2175)
Gene Description:
FA complementation group A
Transcript:
RefSeq NM_000135.2 (NON-CURRENT)
Match location:
Position 1834 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000296800)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACTCCCGTGTGGCGTTTATA
Hairpin Sequence:
5'-CCGG-GACTCCCGTGTGGCGTTTATA-CTCGAG-TATAAACGCCACACGGGAGTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACTCCCGTGTGGCGTTTATACTCGAGTATAAACGCCACACGGGAGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACTCCCGTGTGGCGTTTATACTCGAGTATAAACGCCACACGGGAGTC-3'

Other clones with same target sequence:

(none)