Construct: shRNA TRCN0000296821

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_052934.3-827s21c1
DNA Barcode:
TCTTTACCTTCATTGACATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SLC26A9 (115019)
Gene Description:
solute carrier family 26 member 9
Transcript:
RefSeq NM_052934.3 (NON-CURRENT)
Match location:
Position 827 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000296821)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCTTTACCTTCATTGACATTT
Hairpin Sequence:
5'-CCGG-TCTTTACCTTCATTGACATTT-CTCGAG-AAATGTCAATGAAGGTAAAGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCTTTACCTTCATTGACATTTCTCGAGAAATGTCAATGAAGGTAAAGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCTTTACCTTCATTGACATTTCTCGAGAAATGTCAATGAAGGTAAAGA-3'

Other clones with same target sequence:

(none)