Construct: shRNA TRCN0000296825

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_012428.2-650s21c1
DNA Barcode:
CACCAGTGAAGAGGTCATTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NPTN (27020)
Gene Description:
neuroplastin
Transcript:
RefSeq NM_012428.2 (NON-CURRENT)
Match location:
Position 650 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000296825)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACCAGTGAAGAGGTCATTAT
Hairpin Sequence:
5'-CCGG-CACCAGTGAAGAGGTCATTAT-CTCGAG-ATAATGACCTCTTCACTGGTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACCAGTGAAGAGGTCATTATCTCGAGATAATGACCTCTTCACTGGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACCAGTGAAGAGGTCATTATCTCGAGATAATGACCTCTTCACTGGTG-3'

Other clones with same target sequence:

  1. TRCN0000247863