Construct: shRNA TRCN0000296851

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004973.2-3992s21c1
DNA Barcode:
CAACGCCCGTGGTCGATTTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
JARID2 (3720)
Gene Description:
jumonji and AT-rich interaction domain containing 2
Transcript:
RefSeq NM_004973.2 (NON-CURRENT)
Match location:
Position 3992 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000296851)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAACGCCCGTGGTCGATTTAT
Hairpin Sequence:
5'-CCGG-CAACGCCCGTGGTCGATTTAT-CTCGAG-ATAAATCGACCACGGGCGTTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAACGCCCGTGGTCGATTTATCTCGAGATAAATCGACCACGGGCGTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAACGCCCGTGGTCGATTTATCTCGAGATAAATCGACCACGGGCGTTG-3'

Other clones with same target sequence:

(none)