Construct: shRNA TRCN0000296940

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014899.3-2633s21c1
DNA Barcode:
CTGTTGCTTTAGTGATATTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RHOBTB3 (22836)
Gene Description:
Rho related BTB domain containing 3
Transcript:
RefSeq NM_014899.3 (NON-CURRENT)
Match location:
Position 2633 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000296940)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGTTGCTTTAGTGATATTTA
Hairpin Sequence:
5'-CCGG-CTGTTGCTTTAGTGATATTTA-CTCGAG-TAAATATCACTAAAGCAACAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGTTGCTTTAGTGATATTTACTCGAGTAAATATCACTAAAGCAACAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGTTGCTTTAGTGATATTTACTCGAGTAAATATCACTAAAGCAACAG-3'

Other clones with same target sequence:

(none)