Construct: shRNA TRCN0000297027

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001111116.1-178s21c1
DNA Barcode:
AGTCCCGCTTCTGGTACTTTG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gm15427 (100041686)
Gene Description:
predicted pseudogene 15427
Transcript:
RefSeq NM_001111116.1 (NON-CURRENT)
Match location:
Position 178 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000297027)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGTCCCGCTTCTGGTACTTTG
Hairpin Sequence:
5'-CCGG-AGTCCCGCTTCTGGTACTTTG-CTCGAG-CAAAGTACCAGAAGCGGGACT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGTCCCGCTTCTGGTACTTTGCTCGAGCAAAGTACCAGAAGCGGGACTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGTCCCGCTTCTGGTACTTTGCTCGAGCAAAGTACCAGAAGCGGGACT-3'

Other clones with same target sequence:

  1. TRCN0000438128