Construct: shRNA TRCN0000297059

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006278.1-357s21c1
DNA Barcode:
CCTGCGGCTTGAGGATTATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ST3GAL4 (6484)
Gene Description:
ST3 beta-galactoside alpha-2,3-sialyltransferase 4
Transcript:
RefSeq NM_006278.1 (NON-CURRENT)
Match location:
Position 357 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000297059)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGCGGCTTGAGGATTATTT
Hairpin Sequence:
5'-CCGG-CCTGCGGCTTGAGGATTATTT-CTCGAG-AAATAATCCTCAAGCCGCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGCGGCTTGAGGATTATTTCTCGAGAAATAATCCTCAAGCCGCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGCGGCTTGAGGATTATTTCTCGAGAAATAATCCTCAAGCCGCAGG-3'

Other clones with same target sequence:

  1. TRCN0000005578