Construct: shRNA TRCN0000297101

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133766.3-4103s21c1
DNA Barcode:
GCTGCTGATTAAGTGCAGATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Efr3a (76740)
Gene Description:
EFR3 homolog A
Transcript:
RefSeq NM_133766.3 (CURRENT)
Match location:
Position 4103 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000297101)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGCTGATTAAGTGCAGATA
Hairpin Sequence:
5'-CCGG-GCTGCTGATTAAGTGCAGATA-CTCGAG-TATCTGCACTTAATCAGCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGCTGATTAAGTGCAGATACTCGAGTATCTGCACTTAATCAGCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGCTGATTAAGTGCAGATACTCGAGTATCTGCACTTAATCAGCAGC-3'

Other clones with same target sequence:

  1. TRCN0000175694