Construct: shRNA TRCN0000297172

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_198294.2-4100s21c1
DNA Barcode:
CCACTTCCAAGCCTGATATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tanc1 (66860)
Gene Description:
tetratricopeptide repeat, ankyrin repeat and coiled-coil containing 1
Transcript:
RefSeq NM_198294.2 (NON-CURRENT)
Match location:
Position 4100 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000297172)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACTTCCAAGCCTGATATTT
Hairpin Sequence:
5'-CCGG-CCACTTCCAAGCCTGATATTT-CTCGAG-AAATATCAGGCTTGGAAGTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACTTCCAAGCCTGATATTTCTCGAGAAATATCAGGCTTGGAAGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACTTCCAAGCCTGATATTTCTCGAGAAATATCAGGCTTGGAAGTGG-3'

Other clones with same target sequence:

  1. TRCN0000178631