Construct: shRNA TRCN0000297274

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002721.4-1026s21c1
DNA Barcode:
GCTTCGATCATGGTCTTCAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PPP6C (5537)
Gene Description:
protein phosphatase 6 catalytic subunit
Transcript:
RefSeq NM_002721.4 (NON-CURRENT)
Match location:
Position 1026 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000297274)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTCGATCATGGTCTTCAAA
Hairpin Sequence:
5'-CCGG-GCTTCGATCATGGTCTTCAAA-CTCGAG-TTTGAAGACCATGATCGAAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTCGATCATGGTCTTCAAACTCGAGTTTGAAGACCATGATCGAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTCGATCATGGTCTTCAAACTCGAGTTTGAAGACCATGATCGAAGC-3'

Other clones with same target sequence:

  1. TRCN0000002767