Construct: shRNA TRCN0000297307

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028881.1-751s21c1
DNA Barcode:
GATGCTAAAGTCCCTGCTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Crtc2 (74343)
Gene Description:
CREB regulated transcription coactivator 2
Transcript:
RefSeq NM_028881.1 (NON-CURRENT)
Match location:
Position 751 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000297307)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATGCTAAAGTCCCTGCTATT
Hairpin Sequence:
5'-CCGG-GATGCTAAAGTCCCTGCTATT-CTCGAG-AATAGCAGGGACTTTAGCATC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATGCTAAAGTCCCTGCTATTCTCGAGAATAGCAGGGACTTTAGCATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATGCTAAAGTCCCTGCTATTCTCGAGAATAGCAGGGACTTTAGCATC-3'

Other clones with same target sequence:

  1. TRCN0000176130