Construct: shRNA TRCN0000297333

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005067.5-1078s21c1
DNA Barcode:
CATACGGAGAAACCAGAACAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SIAH2 (6478)
Gene Description:
siah E3 ubiquitin protein ligase 2
Transcript:
RefSeq NM_005067.5 (NON-CURRENT)
Match location:
Position 1078 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000297333)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATACGGAGAAACCAGAACAT
Hairpin Sequence:
5'-CCGG-CATACGGAGAAACCAGAACAT-CTCGAG-ATGTTCTGGTTTCTCCGTATG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATACGGAGAAACCAGAACATCTCGAGATGTTCTGGTTTCTCCGTATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATACGGAGAAACCAGAACATCTCGAGATGTTCTGGTTTCTCCGTATG-3'

Other clones with same target sequence:

  1. TRCN0000007417