Construct: shRNA TRCN0000297446

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020992.2-1238s21c1
DNA Barcode:
GCCTTGGTTAATTGACTCACA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PDLIM1 (9124)
Gene Description:
PDZ and LIM domain 1
Transcript:
RefSeq NM_020992.2 (NON-CURRENT)
Match location:
Position 1238 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000297446)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTTGGTTAATTGACTCACA
Hairpin Sequence:
5'-CCGG-GCCTTGGTTAATTGACTCACA-CTCGAG-TGTGAGTCAATTAACCAAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTTGGTTAATTGACTCACACTCGAGTGTGAGTCAATTAACCAAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTTGGTTAATTGACTCACACTCGAGTGTGAGTCAATTAACCAAGGC-3'

Other clones with same target sequence:

  1. TRCN0000161271