Construct: shRNA TRCN0000297578

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153713.1-1230s21c1
DNA Barcode:
CCTATTTGCTAAGAGATCCCT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LIX1L (128077)
Gene Description:
limb and CNS expressed 1 like
Transcript:
RefSeq NM_153713.1 (NON-CURRENT)
Match location:
Position 1230 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000297578)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTATTTGCTAAGAGATCCCT
Hairpin Sequence:
5'-CCGG-CCTATTTGCTAAGAGATCCCT-CTCGAG-AGGGATCTCTTAGCAAATAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTATTTGCTAAGAGATCCCTCTCGAGAGGGATCTCTTAGCAAATAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTATTTGCTAAGAGATCCCTCTCGAGAGGGATCTCTTAGCAAATAGG-3'

Other clones with same target sequence:

  1. TRCN0000135165