Construct: shRNA TRCN0000297579

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153713.1-715s21c1
DNA Barcode:
CCTTCCGATTCATGCTGGAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LIX1L (128077)
Gene Description:
limb and CNS expressed 1 like
Transcript:
RefSeq NM_153713.1 (NON-CURRENT)
Match location:
Position 715 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000297579)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTCCGATTCATGCTGGAAT
Hairpin Sequence:
5'-CCGG-CCTTCCGATTCATGCTGGAAT-CTCGAG-ATTCCAGCATGAATCGGAAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTCCGATTCATGCTGGAATCTCGAGATTCCAGCATGAATCGGAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTCCGATTCATGCTGGAATCTCGAGATTCCAGCATGAATCGGAAGG-3'

Other clones with same target sequence:

  1. TRCN0000138619