Construct: shRNA TRCN0000297753

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_170709.1-838s21c1
DNA Barcode:
GGCTGAACGTAATGTGCTCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SGK3 (23678)
Gene Description:
serum/glucocorticoid regulated kinase family member 3
Transcript:
RefSeq NM_170709.1 (NON-CURRENT)
Match location:
Position 838 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000297753)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGCTGAACGTAATGTGCTCTT
Hairpin Sequence:
5'-CCGG-GGCTGAACGTAATGTGCTCTT-CTCGAG-AAGAGCACATTACGTTCAGCC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGCTGAACGTAATGTGCTCTTCTCGAGAAGAGCACATTACGTTCAGCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGCTGAACGTAATGTGCTCTTCTCGAGAAGAGCACATTACGTTCAGCC-3'

Other clones with same target sequence:

  1. TRCN0000010282