Construct: shRNA TRCN0000297833

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004862.3-397s21c1
DNA Barcode:
GCATGAATCCTCCTTCGTATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LITAF (9516)
Gene Description:
lipopolysaccharide induced TNF factor
Transcript:
RefSeq NM_004862.3 (CURRENT)
Match location:
Position 397 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000297833)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATGAATCCTCCTTCGTATT
Hairpin Sequence:
5'-CCGG-GCATGAATCCTCCTTCGTATT-CTCGAG-AATACGAAGGAGGATTCATGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATGAATCCTCCTTCGTATTCTCGAGAATACGAAGGAGGATTCATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATGAATCCTCCTTCGTATTCTCGAGAATACGAAGGAGGATTCATGC-3'

Other clones with same target sequence:

  1. TRCN0000013459