Construct: shRNA TRCN0000297844

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020899.3-1440s21c1
DNA Barcode:
GAGACCTTTGTCACTTACTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZBTB4 (57659)
Gene Description:
zinc finger and BTB domain containing 4
Transcript:
RefSeq NM_020899.3 (NON-CURRENT)
Match location:
Position 1440 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000297844)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGACCTTTGTCACTTACTAT
Hairpin Sequence:
5'-CCGG-GAGACCTTTGTCACTTACTAT-CTCGAG-ATAGTAAGTGACAAAGGTCTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGACCTTTGTCACTTACTATCTCGAGATAGTAAGTGACAAAGGTCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGACCTTTGTCACTTACTATCTCGAGATAGTAAGTGACAAAGGTCTC-3'

Other clones with same target sequence:

  1. TRCN0000015783