Construct: shRNA TRCN0000297887

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022460.3-1151s21c1
DNA Barcode:
CGAGATGGACATCTTGCAGTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HS1BP3 (64342)
Gene Description:
HCLS1 binding protein 3
Transcript:
RefSeq NM_022460.3 (NON-CURRENT)
Match location:
Position 1151 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000297887)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAGATGGACATCTTGCAGTA
Hairpin Sequence:
5'-CCGG-CGAGATGGACATCTTGCAGTA-CTCGAG-TACTGCAAGATGTCCATCTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAGATGGACATCTTGCAGTACTCGAGTACTGCAAGATGTCCATCTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAGATGGACATCTTGCAGTACTCGAGTACTGCAAGATGTCCATCTCG-3'

Other clones with same target sequence:

  1. TRCN0000138279