Construct: shRNA TRCN0000297916

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015345.2-520s21c1
DNA Barcode:
CAGAGTCTGTACGCGTTTGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DAAM2 (23500)
Gene Description:
dishevelled associated activator of morphogenesis 2
Transcript:
RefSeq NM_015345.2 (NON-CURRENT)
Match location:
Position 520 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000297916)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGAGTCTGTACGCGTTTGAT
Hairpin Sequence:
5'-CCGG-CAGAGTCTGTACGCGTTTGAT-CTCGAG-ATCAAACGCGTACAGACTCTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGAGTCTGTACGCGTTTGATCTCGAGATCAAACGCGTACAGACTCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGAGTCTGTACGCGTTTGATCTCGAGATCAAACGCGTACAGACTCTG-3'

Other clones with same target sequence:

  1. TRCN0000158307