Construct: shRNA TRCN0000298012

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011955.1-142s21c1
DNA Barcode:
CGTGGAGGAAATCAGAGAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nubp1 (26425)
Gene Description:
nucleotide binding protein 1
Transcript:
RefSeq NM_011955.1 (NON-CURRENT)
Match location:
Position 142 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000298012)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTGGAGGAAATCAGAGAGAA
Hairpin Sequence:
5'-CCGG-CGTGGAGGAAATCAGAGAGAA-CTCGAG-TTCTCTCTGATTTCCTCCACG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTGGAGGAAATCAGAGAGAACTCGAGTTCTCTCTGATTTCCTCCACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTGGAGGAAATCAGAGAGAACTCGAGTTCTCTCTGATTTCCTCCACG-3'

Other clones with same target sequence:

  1. TRCN0000182112