Construct: shRNA TRCN0000298056

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_080638.2-2083s21c1
DNA Barcode:
CCATCGAAATTACAACCAACT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mvp (78388)
Gene Description:
major vault protein
Transcript:
RefSeq NM_080638.2 (NON-CURRENT)
Match location:
Position 2083 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000298056)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATCGAAATTACAACCAACT
Hairpin Sequence:
5'-CCGG-CCATCGAAATTACAACCAACT-CTCGAG-AGTTGGTTGTAATTTCGATGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATCGAAATTACAACCAACTCTCGAGAGTTGGTTGTAATTTCGATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATCGAAATTACAACCAACTCTCGAGAGTTGGTTGTAATTTCGATGG-3'

Other clones with same target sequence:

  1. TRCN0000175229